Bilirubin reagent is used for determination of Total and Direct Bilirubin based on Jendrassik & Grof method, using Diazotized Sulphonic Acid with DMSO as an activator for Total Bilirubin. Autozyme Bilirubin is a two reagent system using one procedure, can be determined in just 5 minutes. Bilirubin t&d test is usually one of a group of tests to check the health of your liver. Bilirubin testing may be done to Investigate jaundice which is yellowing of the skin & eyes caused by high levels of bilirubin.